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Molecular Probes™ Propidium Iodide - 1.0 mg/mL Solution in Water

Catalog No. P3566
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P3566 10 mL
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Catalog No. P3566 Supplier Molecular Probes™ Supplier No. LSP3566
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Description

Propidium Iodide - 1.0 mg/mL Solution in Water

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population.

PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.

PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.

Learn more about propidium iodide, and propidium iodide containing products

Specifications

Content And Storage Contains 1 bottle of propidium iodide(1.0 mg/mL solution in water).
Store in refrigerator (2–8°C) and protect from light.
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Propidium Iodide
Dye Type propidium iodide
Emission 533/617
Form Solution
Quantity 10 mL
Shipping Condition Room Temperature
Sub Cellular Localization Cytoplasm & Cytosol
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Frequently Asked Questions (FAQs)

I have a LIVE/DEAD BacLight Bacterial Viability kit that has SYTO 9 and propidium iodide in it. Will I be able to stain eukaryotic cells that have engulfed bacteria and determine if the bacteria are alive or dead using this kit?

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.
Is propidium iodide (PI) fixable with glutaraldehyde or paraformaldehyde (PFA)?

PI is not fixable with glutaraldehyde or PFA. Both reagents fix by crosslinking amines. PI and other nucleic acid stains do not inherently bind covalently to nucleic acids and these fixatives do not crosslink the dyes to nucleic acids.
The one fixable nucleic acid stain is Ethidium Monoazide Bromide (EMA), Cat no. E1374); it covalently binds to nucleic acids upon activation by exposure to light.

For Research Use Only. Not for use in diagnostic procedures.