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Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.
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2

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Components >50% Water, 25 to 50% Glycerin
Cut Site AT.CGAT
Content And Storage Keep container tightly closed
Form Liquid
Product Type ClaI (Bsu15I)
Storage Buffer 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
5

OPTIZYME™ BglII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site A.GATCT
Content And Storage Keep container tightly closed
Form Liquid
Product Type BglII
Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
7

OPTIZYME™ SmaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site CCC.GGG
Form Liquid
Product Type SmaI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 30°C
Concentration 10 U/μL
For Use With (Application) >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI
9

OPTIZYME™ NotI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site GC.GGCCGC
Content And Storage Keep container tightly closed
Form Liquid
Product Type NotI
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Concentration 10 U/μL
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
10

OPTIZYME™ HindIII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site A.AGCTT
Content And Storage Keep container tightly closed
Form Liquid
Product Type HindIII
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
13

Thermo Scientific™ Tru1I (MseI), HC (50 U/μL)

Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).

14

Thermo Scientific™ VspI (AseI) (10 U/μL)

Cut at AT^TAAT sites with VspI (AseI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AseI, PshBI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme VspI (AseI)
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer O
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 10 U/μL
15

Thermo Scientific™ BplI (5 U/μL)

Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer.

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme BplI
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X Buffer Tango
Type IIS RE No
Optimal Reaction Temperature 37°C
Concentration 5 U/μL