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OPTIZYME™ HinfI, Fisher BioReagents™

Supplier:  Fisher BioReagents BP80511

Catalog No. BP8051-1



Description

Description

5'...G^A N T C...3'
3'...C T N A^G...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 50 - 100%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with HinfI.
  • Compatible Ends: G^AWTC – PfeI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - cleavage impaired
    • 5'...GANTm5C G...3'
  • 3'...CTNA Gm5C...5'
  • (Cleavage is impaired)
    • 5'...m5C GANTm5C G...3'
  • 3'... Gm5CTNA Gm5C...5'
  • (Cleavage is impaired)
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - blocked
    • 5'...TGm6ANTC(N)5 TGCT...3'
  • 3'...AC TNAG(N)5m6ACGA...5'
  • (Cleavage is blocked)
  • Specifications

    Specifications

    10 U/μL
    37°C
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    2000 U
    HinfI
    25 to 50% Water, >50% Glycerin
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
    Keep container tightly closed
    G.ANTC
    Liquid
    SDS
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