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OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

Supplier:  Fisher BioReagents BP80581

Catalog No. BP8058-1



Description

Description

5'...A T G C A^T...3'
3'...T^A C G T A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 – 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 50 - 100%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NsiI.
  • Compatible Ends: Alw21I, PstI, SdaI, SduI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined
  • Specifications

    Specifications

    10 U/μL
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    1000 U
    NsiI
    25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
    >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
    Keep container tightly closed
    ATGCA.T
    Liquid
    SDS
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