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OPTIZYME™ BglI, Fisher BioReagents™

Catalog No. BP80461
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Quantity:
2000 U
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Catalog No. Quantity
BP80461 2000 U
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Catalog No. BP80461 Supplier Thermo Fisher Scientific Supplier No. BP8046 1
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Description

5'...G C C N N N N^N G G C...3'
3'...C G G N^N N N N C C G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 50 - 100%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BglI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: May overlap - no effect
    • 5'...GCm5CW GGNNGGC...3'
  • 3'...CG GWm5CCNNCCG...5'
  • (No effect on cleavage)
  • CpG: May overlap - cleavage impaired
    • 5'...GCm5C G(N)3m5C GGC...3'
  • 3'...CG Gm5C(N)3 Gm5CCG...5'
  • (No effect on cleavage)
    • 5'...GCC(N)5GGm5C G...3'
  • 3'...CGG(N)5CC Gm5C...5'
  • (Cleavage is impaired)
    • 5'...m5C GCC(N)5GGm5C G...3'
  • 3'... Gm5CGG(N)5CC Gm5C...5'
  • (Cleavage is impaired)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglI
    Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 2000 U
    Cut Site GCCNNNN.NGGC
    Product Type BglI
    Form Liquid
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