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OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™
Supplier: Thermo Fisher Scientific BP80241
Description
5'...A T^C G A T...3'
3'...T A G C^T A...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 20 - 50%
- Buffer 2: 20 - 50%
- Buffer 3: 20 - 50%
- Buffer 4: 100%
- Buffer 5: 20 - 50%
- 10mM Tris-HCl (pH 8.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycero
- ClaI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain.
- 5'...ATCGm6A TC...3'
- 5'...ATCGAT...3'
Greener Choice Claims
- Environmental benefits include:
- Non-Toxic
Specifications
| 10 U/μL | |
| 37°C | |
| >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Cfr9I | |
| Keep container tightly closed | |
| AT.CGAT | |
| Liquid |
| >50% Water, 25 to 50% Glycerin | |
| 7.4 | |
| 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol | |
| 600 U | |
| ClaI (Bsu15I) |