missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. OPTIZYME™ BglII, Fisher BioReagents™

OPTIZYME™ BglII, Fisher BioReagents™

Catalog No. BP80145
Change view
Click to view available options
Quantity:
2500 U
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
BP80145 2500 U
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. BP80145 Supplier Thermo Fisher Scientific Supplier No. BP8014 5
Only null left

Description

5'...A^G A T C T...3'
3'...T C T A G^A...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: 0 - 20%
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with BglII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BglII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: BamHI, BclI, Bsp143I, MboI, PsuI
  • Methylation Effects:
  • Dam: Completely overlaps - no effect
    • AGm6ATCT
  • (No effect on cleavage)
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - cleavage impaired
    • 5'...TGm6AGATCT(N)3 TGCT...3'
  • 3'...AC TCTAGA(N)3m6ACGA...5'
  • (Cleavage is impaired)

    • Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
    Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 2500 U
    Cut Site A.GATCT
    Product Type BglII
    Form Liquid
    Show More Show Less