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OPTIZYME™ MspI (HpaII), Fisher BioReagents™

Supplier:  Fisher BioReagents BP80481

Catalog No. BP8048-1



Description

Description

5'...C^C G G...3'
3'...G G C^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with MspI.
  • Note:
    • When the external C in the sequence: CCGG is methylated, both MspI and HpaII cannot cleave. MspI is an isoschizomer of HpaII. However, unlike HpaII, MspI can cleave the sequence when the internal C residue is methylated.
  • Compatible Ends: Bsp119I, Bsu15I, Hin1I, Hin6I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - no effect
    • CCGG
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect
  • Specifications

    Specifications

    10 U/μL
    37°C
    Gene cloning and DNA genotyping
    3000 U
    MspI
    10X OPTIZYME™ Buffer 4
    7.4
    10mM Potassium Phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    C.CGG
    Liquid
    SDS
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