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OPTIZYME™ BpiI (BbsI), Fisher BioReagents™

Catalog No. BP80721
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1000 U
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Catalog No. Quantity
BP80721 1000 U
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Catalog No. BP80721 Supplier Thermo Fisher Scientific Supplier No. BP8072 1
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Description

5'...G A A G A C (N)2^...3'
3'...C T T C T G (N)6^...5'

Supplied with: 10X OPTIZYME Buffer 2
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 2: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 100%
  • Buffer 3: 50 - 100%
  • Buffer 4: 50 - 100%
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BpiI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BpiI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note:
    • BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for direct PCR product cloning.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - no effect
    • 5'...GAAGAm5C G...3'
  • 3'...CTTCT Gm5C...5'
  • (No effect on cleavage)
    • 5'...m5C GAAGAC...3'
  • 3'... Gm5CTTCTG...5'
  • (No effect on cleavage)
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined

    • Specifications

    Concentration 10 U/μL
    Components 25 to 50% Water, >50% Glycerin
    Incubator Temperature 37°C
    pH 7.4
    For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BpI
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    Content And Storage Keep container tightly closed
    Quantity 1000 U
    Cut Site GAAGAC(2/6).
    Product Type BpI
    Form Liquid
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