Competent Cells
MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
Invitrogen™ MultiShot™ StripWell BL21 Star™ (DE3) Competent Cells
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.
Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
MilliporeSigma™ Annexin V-PE Apoptosis Detection Kit
Identifies phosphatidylserine (PS) that translocates from cytoplasmic face of plasma membrane to cell surface during apoptosis.
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli
One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.
MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.
MilliporeSigma™ Novagen™ Rosetta™ Competent Cells
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Invitrogen™ MAX Efficiency™ DH5α-T1R Competent Cells
MAX Efficiency DH5α T1R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5.
Invitrogen™ Tali™ Calibration Beads
Align and calibrate Tali™ Image-Based Cytometer every three months