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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ Tali™ Cell Cycle Kit  DFS Item

Convenient and accurate determination of the percentage of cells in each phase of the cell cycle

2

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
3

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

4

MilliporeSigma™ Novagen™ Origami™ 2(DE3) Singles™ Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Efficacité Guaranteed efficiency: 2 x 106 cfu/μg
Contenu et stockage ≤-70°C
5

MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells

Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
6

MilliporeSigma™ Novagen™ Rosetta™ Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
7

MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells

NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.

Efficacité Guaranteed efficiency: >1 x 109 cfu/μg
Contenu et stockage ≤-70°C
8

MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Efficacité Guaranteed efficiency: >2 x 106 cfu/μg
Contenu et stockage ≤-70°C
9

Invitrogen™ MAX Efficiency™ DH5α-T1R Competent Cells  DFS Item

MAX Efficiency DH5α T1R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5.

10

Invitrogen™ Tali™ Viability Kit - Dead Cell Red  DFS Item

Validated for use with the Tali Image-Based Cytometer

11

Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.

Résistance aux antibiotiques des bactéries Yes (Streptomycin)
Clonage d’ADN méthylé Yes (mcrA)
Type de produit Electrocompetent Cells
Plasmide May be used for plasmids >20 kb
Résistant au phage T1 (tonA) No
Espèces E. coli (K12)
Clonage d’ADN instable No
Conditions d’expédition Dry Ice
Compatibilité à haut débit Low
Format Tube
Sélection bleue / blanche Yes (lacZΔM15)
Niveau d’efficacité de la transformation High Efficiency (>1 x 1010 cfu/μg)
Gamme de produits One Shot
Contient l’épisome F No
Réduit la recombinaison Yes (recA13)
Améliore la qualité des plasmides Yes (endA1)
Préparation de l’ADN non méthylé No
12

MilliporeSigma™ Novagen™ NovaBlue Singles™ Competent Cells

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

Efficacité Guaranteed efficiency: >1.5 x 108 cfu/μg
Contenu et stockage ≤-70°C
13

Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically Competent E. coli  DFS Item

One Shot BL21 Star (DE3)pLysS Chemically Competent E. coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET.

14

Gibco™ Expi293™ Met (–) Expression Medium  DFS Item

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.

15

MilliporeSigma™ Novagen™ RosettaBlue™ (DE3)pLysS Competent Cells

RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Efficacité Guaranteed efficiency: >1 x 106 cfu/μg
Contenu et stockage ≤-70°C