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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ One Shot™ BL21(DE3)pLysE Chemically Competent E. coli  DFS Item

One Shot BL21(DE3)pLysE Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

2

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

3

Invitrogen™ Tali™ Calibration Beads  DFS Item

Align and calibrate Tali™ Image-Based Cytometer every three months

4

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells  DFS Item

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

5

Invitrogen™ One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells  DFS Item

One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection.

6

Gibco™ FreeStyle™ CHO Expression Medium  DFS Item
GREENER_CHOICE

Animal origin-free, chemically defined, protein-free medium

Cell Line FreeStyle™ CHO-S Cells, CHO
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Product Type Expression Medium
Endotoxin Level ≤0.25 EU/mL, ≤ 0.25 EU/mL
Classification Chemically Defined, Protein-Free, Serum-Free
Culture Type Suspension Cell Culture
Phenol Red Indicator No Phenol Red
Product Line Freestyle, Gibco
Serum Level Serum-Free
Glutamine No Glutamine
7

MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Content And Storage ≤-70°C
8

MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >5 x 106 cfu/μg
9

MilliporeSigma™ Novagen™ RosettaBlue™ (DE3)pLysS Competent Cells

RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 106 cfu/μg
10

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

11

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
12

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

13

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome Yes
14

Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells  DFS Item

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

15

Invitrogen™ MultiShot™ FlexPlate DH5α T1R Competent Cells  DFS Item

MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications.