Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (Cat. No. K0491, K0492) and may be purchased separately.
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents.