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Thermo Scientific™ RNA Gel Loading Dye (2X)

Catalog No. FERR0641
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FERR0641 1 mL
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Catalog No. FERR0641 Supplier Thermo Scientific™ Supplier No. R0641
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Description

Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.

2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels. It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide. In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.

2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilizes RNA.

Highlights

  • Two-color tracking of DNA, RNA migration during electrophoresis
  • No DNA, RNA masking during gel exposure to UV light
  • Denaturation of DNA, RNA due to the presence of formamide

Specifications

Concentration 2X
Sample Type RNA
Gel Compatibility Acrylamide Gels, Agarose Gels
Label or Dye Bromophenol Blue, Xylene Cyanol FF (XCFF)
Product Type RNA Gel Loading Dye
Quantity 1 mL

Frequently Asked Questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
Can I use Thermo Scientific 2X RNA Loading Dye for native gel electrophoresis?

Thermo Scientific 2X RNA Loading Dye contains 95% formamide as a denaturing agent and hence cannot be used for native gel electrophoresis.

For Research Use Only. Not for use in diagnostic procedures.