Miscellaneous Molecular Biology Reagents
MilliporeSigma™ Calbiochem™ DNase I, Bovine Pancreas
Acts on single and double stranded DNA
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Invitrogen™ DNase I Buffer (10X)
An Invitrogen component of the MAXIscript™ Kits, the buffer is supplied in one tube containing 1 mL.
Invitrogen™ dNTP Mix (2.5 mM)
The same high-quality deoxynucleotide triphosphate solutions provided in the Invitrogen™ RETROscript™ First Strand cDNA Synthesis Kit for RT-PCR.
Pharmalytes™ pH range 4.0 - 6.5, MilliporeSigma™ Supelco™
Pharmalytes™ are ampholytes which are used to create a pH gradient under application of an electric field.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Polysorbate 80
CAS: 9005-65-6 Synonym: Polyoxyethylene(20)sorbitan monooleate
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Invitrogen™ MagMAX™ Wash Solution 1 Concentrate
Invitrogen MagMAX Wash Solution 1 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
Invitrogen™ Yeast RNA (5 mg/mL)
Purified Saccharomyces cerevisiae yeast RNA is is a very effective blocking agent when used in Northern prehybridization and hybridization buffers, as well as being suitable as a coprecipitant in nucleic acid precipitations.
Invitrogen™ Yeast RNA (10 mg/mL)
Purified Torulla Invitrogen yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
Thermo Scientific™ Agarose I (Molecular Biology Grade)
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.
MilliporeSigma™ Calbiochem™ Hypoxanthine
Used in conjunction with thymidine and aminopterin for cloning and selection of hybridoma cell lines
MilliporeSigma™ Calbiochem™ Ethidium Bromide Adsorber
Safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions
Invitrogen™ Yeast tRNA (10 mg/mL)
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.