Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.