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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Format Kit
Type de produit Cloning Kit
Souche bactérienne/de levure TOP10
Type de cellules Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
2

Invitrogen™ TOPO™ TA, Dual TOP10 (Supply Center Packaging)  DFS Item

For fast, efficient cloning and subsequent in vitro transcription/translation

3

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

For fast, efficient cloning and subsequent in vitro transcription/translation

Promoteur T7, SP6
Format Kit
Type de produit Cloning Kit
Souche bactérienne/de levure TOP10
Type de cellules Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
5

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Promoteur T7, SP6
Format Kit
Type de produit Cloning Kit
Comprend Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Souche bactérienne/de levure TOP10F ́
Type de cellules Chemically Competent E. coli
Vector pCRII
Méthode de clonage TA Cloning
Gamme de produit One Shot
À utiliser avec (application) PCR Cloning
Contenu et stockage This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
6

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning  DFS Item

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

7

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Promoteur T7, T3
Format Kit
Type de produit Cloning Kit
Souche bactérienne/de levure DH5α
Vector TOPO-TA Cloning Vectors
Méthode de clonage TOPO™-TA
Gamme de produit One Shot
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
8

Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit  DFS Item

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

9

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Promoteur T7, SP6
Format Kit
Type de produit Cloning Kit
Souche bactérienne/de levure DH5α
Vector TOPO-TA Cloning Vectors
Méthode de clonage TOPO™-TA
Gamme de produit One Shot
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
10

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells  DFS Item

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Shipping condition (Condition d'expédition) Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoteur T3, T7
Format Kit
Type de produit Cloning Kit
Résistance aux antibiotiques bactérienne Ampicillin (AmpR), Kanamycin (KanR)
Souche bactérienne/de levure OmniMAX2
Type de cellules Chemically Competent E. coli
Vector pCR-XL-2-TOPO
Méthode de clonage Zero Blunt TOPO
Gamme de produit TOPO
À utiliser avec (application) PCR Cloning
Contenu et stockage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:
• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Store competent cells and pUC19 at -65 to -85°C.
11

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process

12

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells  DFS Item

Format Kit
Type de produit Cloning Kit
Résistance aux antibiotiques bactérienne Ampicillin (AmpR), Kanamycin (KanR)
Méthode de clonage TOPO™-TA
Gamme de produit TOPO
13

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit  DFS Item

Provides a 5 minute, one-step cloning strategy. Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR4-TOPO Vector, One Shot™ TOP10 Chemically Competent E. coli and PureLink Plasmid Prep Kit enables direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.