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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (238)
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2

Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers  DFS Item

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.

Accuracy 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
Product Type Polymer
For Use With (Application) Standard Sequencing, Fragment Analysis (Denaturing)
Test Time 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Product Line POP-7
3

Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit  DFS Item

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
5

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix  DFS Item

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Shipping Condition Ambient, Wet Ice, Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 6 fragments
Product Type HiFi Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
6

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control DNA  DFS Item

VetMAX™ Xeno™ Internal Positive Control (IPC) DNA serves as an internal positive control for the DNA purification process and helps monitor for the presence of PCR inhibitors in animal health molecular detection workflows. This is of particular importance when working with sample types that have a high level of inhibitors such as feces.

Shipping Condition Dry Ice
Includes Internal Positive Control
Format Kit
Product Type Internal Positive Control DNA
For Use With (Application) Detection and Identification of Animal Disease
Species Not Species-Specific
Product Line VetMAX, Xeno
7

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells  DFS Item

Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
8

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning  DFS Item

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

9

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Vector TOPO-TA Cloning Vectors
Product Line One Shot
10

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10F´
Vector TOPO-TA Cloning Vectors
Product Line One Shot
Cell Type Chemically Competent
11

Invitrogen™ GeneRacer™ Kit with AMV RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

12

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process

13

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

14

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit  DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

15

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL  DFS Item

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor