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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
Séquençage de l’ADN (221)
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Kits de clonage par PCR (104)
Clonage d’ADN, cartographie et mutagenèse (83)
ADN plasmide (43)

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1

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL  DFS Item

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

2

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits One Shot
Type de cellule Chemically Competent
Accélérateur T7, T3
Méthode de clonage Blunt TOPO™
À utiliser avec (application) Chromatin Biology
3
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

4

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Offers the fastest and easiest method for high-efficiency cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits One Shot
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
5

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

6

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells  DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Souche bactérienne ou de levures Not Included
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits TOPO, Zero Blunt
Accélérateur T7
Méthode de clonage Blunt PCR
Contenu et stockage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
8

Invitrogen™ Zero Blunt™ PCR Cloning Kit  DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits Zero Blunt
Accélérateur T7
Méthode de clonage Blunt PCR
Contenu et stockage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
9

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit  DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

10

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Type de cellule Chemically Competent
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
11

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Type de cellule Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
12

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
13

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli  DFS Item

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

14

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Each kit uses the pCR™4Blunt-TOPO; vector with specially designed sequencing primer sites that return more insert sequence and less vector sequence from each reaction

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, T3
Méthode de clonage Blunt TOPO™
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
15

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli  DFS Item

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System