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  6. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10F' Chemically Competent E. coli

Catalog No. K203040
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40 Reactions
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Catalog No. No. of Reactions
K203040 40 Reactions
K203001 20 Reactions
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Catalog No. K203040 Supplier Invitrogen™ Supplier No. K203040
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Description

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

  • Fast and convenient: 15-minute, room-temperature ligation
  • Efficient: blue/white screening and >80% clones with correct insert
  • Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
  • Hassle-free: eliminates any enzymatic modifications of PCR product
  • Streamlined: does not require use of PCR primers that contain restriction sites
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • T7 promoter for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • M13 forward and reverse primer sites for sequencing

  • How TA Cloning™ Works
  • Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
  • Llinearized vector supplied in this kit has single 3' deoxythymidine (T) residues
  • Allows PCR inserts to ligate efficiently with vector
  • Kit Configurations
  • TA Cloning™ Kit is offered in a variety of configurations: without competent cells (K2020-20 and K2020-40), with One Shot™ INVF' Chemically Competent E. coli (K2000-01 and K2000-40), with One Shot™ TOP10F' Chemically Competent E. coli (K2030-01 and K2030-40), and with One Shot™ TOP10 Chemically Competent E. coli (K2040-01 and K2040-40) in 20- and 40-reaction kit sizes

Cloning, PCR Cloning

Specifications

Bacterial or Yeast Strain TOP10F ́
Cell Type Chemically Competent E. coli
Cloning Method TA Cloning
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 40 Reactions
Product Line One Shot
Product Type Cloning Kit
Promoter T7
Quantity 40 reactions
Vector pCR2.1
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For Research Use Only. Not for use in diagnostic procedures.