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Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Catalog No. 12535029
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Catalog No. 12535029 Supplier Invitrogen™ Supplier No. 12535029
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Description

Includes

Gateway BP Clonase II enzyme mix and reaction buffer, Gateway pDONR221 or pDONR/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and OmniMAX2 Competent Cells.

Enables rapid cloning of one or more genes into virtually any protein expression system

  • Designed to create entry clone following PCR with attB-modified custom primers
  • With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
  • PCR product is directionally cloned with efficiencies typically >99%
  • Versatility to use any amplification enzyme
  • Cloning without need for restriction endonucleases or ligase
  • Selection of entry clones with kanamycin or Zeocin™
  • In addition, pDONR221 and pDONR/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use

Cloning, Gateway Cloning

Specifications

Cloning Method Gateway™
Content And Storage The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.
No. of Reactions 20 reactions
Product Line One Shot
Product Type PCR Cloning System
Quantity 20 Reactions
Vector pDONR

For Research Use Only. Not for use in diagnostic procedures.