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Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells 
Enables rapid cloning of one or more genes into virtually any protein expression system
Supplier: Invitrogen™ 12535029
Includes: Gateway BP Clonase II enzyme mix and reaction buffer, Gateway pDONR221 or pDONR/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and OmniMAX2 Competent Cells.
Description
- Designed to create entry clone following PCR with attB-modified custom primers
- With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
- PCR product is directionally cloned with efficiencies typically >99%
- Versatility to use any amplification enzyme
- Cloning without need for restriction endonucleases or ligase
- Selection of entry clones with kanamycin or Zeocin™
- In addition, pDONR221 and pDONR/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use
Cloning, Gateway Cloning
Specifications
| Gateway™ | |
| 20 reactions | |
| PCR Cloning System | |
| pDONR |
| The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C. | |
| One Shot™ | |
| 20 Reactions |
For Research Use Only. Not for use in diagnostic procedures.