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Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Souche bactérienne ou de levures | TOP10 |
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Format | Kit |
Type de produit | Cloning Kit |
Type de cellule | Chemically Competent |
Méthode de clonage | TOPO™-TA |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Résistance aux antibiotiques des bactéries | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Vecteur | TOPO Cloning Vectors |
Gamme de produits | TOPO™ |
Conditions d’expédition | Dry Ice |
Accélérateur | T7, T3 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Résistance aux antibiotiques des bactéries | Kanamycin (KanR), Zeocin™ (ZeoR) |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Accélérateur | SP6 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Souche bactérienne ou de levures | TOP10 |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Type de cellule | Chemically Competent |
Accélérateur | SP6 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Résistance aux antibiotiques des bactéries | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Vecteur | TOPO-TA Vectors |
Gamme de produits | TOPO™ |
Accélérateur | T7, SP6 |
Méthode de clonage | TOPO™-TA |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Enables rapid cloning of one or more genes into virtually any protein expression system
Enables rapid cloning of one or more genes into virtually any protein expression system
Résistance aux antibiotiques des bactéries | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Type de produit | Cloning Kit |
Gamme de produits | TOPO™ |
Méthode de clonage | TOPO™-TA |
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.
Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Souche bactérienne ou de levures | Not Included |
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Format | Kit |
Type de produit | Cloning Kit |
Vecteur | pCR2.1 |
Gamme de produits | TA Cloning™ |
Accélérateur | T7 |
Méthode de clonage | TA Cloning |
À utiliser avec (application) | PCR Cloning |
Contenu et stockage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector
Souche bactérienne ou de levures | INVαF ́ |
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Format | Kit |
Type de produit | Cloning Kit |
Vecteur | pCRII |
Gamme de produits | One Shot™ |
Comprend | Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. |
Type de cellule | Chemically Competent E. coli |
Accélérateur | T7, SP6 |
Méthode de clonage | TA Cloning |
À utiliser avec (application) | PCR Cloning |
Contenu et stockage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System