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Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Content And Storage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPOâ„¢-TA |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Shipping Condition | Dry Ice |
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Promoter | T7, T3 |
Content And Storage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Vector | TOPO Cloning Vectors |
Product Line | TOPOâ„¢ |
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocinâ„¢ (ZeoR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Designed for cloning PCR products directly from a PCR reaction in just 5 minutes
Enables rapid cloning of one or more genes into virtually any protein expression system
Enables rapid cloning of one or more genes into virtually any protein expression system
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPOâ„¢-TA |
Product Line | TOPOâ„¢ |
Used in suspension-adapted cells for transient protein expression. Gibcoâ„¢ pcDNAâ„¢3.4 TOPOâ„¢ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.
Promoter | T7, SP6 |
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Cloning Method | TOPOâ„¢-TA |
Vector | TOPO-TA Vectors |
Product Line | TOPOâ„¢ |