accessibility menu, dialog, popup

UserName

PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
Format 
  • (86)
  • (2)

Filtered Search Results

Products from some of our suppliers do not display in filtered search results. Please clear all filters to see these products.

Narrow Results

Narrow Results

No results found within this category. Try removing some selected filters and try again.

category

special offers

  • (2)

brands

  • (2)
  • (4)
  • (96)
  • (102)

Format

  • (86)
  • (2)
1 – 15 of 70 results
1
Promotions Available

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

2

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Shipping Condition Dry Ice
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Vector TOPO Cloning Vectors
Product Line TOPO
3

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

4

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

5

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

6

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

7

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
10

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

11

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

12

Invitrogen™ RNase T1 (1 U/μL)

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

13

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter T3, T7
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:
• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Store competent cells and pUC19 at -65 to -85°C.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method Zero Blunt TOPO
Bacterial or Yeast Strain OmniMAX2
For Use With (Application) PCR Cloning
Vector pCR-XL-2-TOPO
Product Line TOPO
Cell Type Chemically Competent E. coli
14

Invitrogen™ GeneRacer™ Kit with AMV RT and TOPO TA Cloning™ Kit for Sequencing

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

15

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends