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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

2

Applied Biosystems™ MicroSEQ™ D2 rDNA Fungal PCR Kit
GREENER_CHOICE

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi.

3

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit  DFS Item

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Shipping Condition Room Temperature
Target DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage

The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:
• ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format Kit
Product Type PCR Clean-Up Kit
Sample Type PCR Products
Isolation Technology ChargeSwitch™ Chemistry
Run Time 5 min
For Use With (Application) Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility High-throughput Compatible
Product Line ChargeSwitch
4

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Purity or Quality Grade LD (Low DNA)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Standard
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT, SYBR
5

Invitrogen™ PureLink™ PCR Purification Binding Buffer B2  DFS Item

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

6

Applied Biosystems™ CLA GlobalFiler™ PCR Amplification Kit

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

7

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

8

Applied Biosystems™ SNaPshot™ Multiplex Kit  DFS Item

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Product Type Multiplex Kit
For Use With (Equipment) 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type DNA
Technique SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line SNaPshot
Detection Method Primer-probe
9

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit  DFS Item

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

For Use With (Equipment) 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
10

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
11

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

12

Thermo Scientific™ Mutation Generation System Kit

Perform functional analysis of proteins with these transposon-based tools.

13

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

14

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
15

Invitrogen™ PureLink™ Pro 96 PCR Purification Kit  DFS Item

The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.