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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1 – 15 of 58 results
1

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

2

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
3

Invitrogen™ Precision gRNA Synthesis Kit  DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

4

Invitrogen™ RNA Control 250 with Manual  DFS Item

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Content And Storage RNA Control and THE RNA Storage Solution should be stored at –20°C
Format Tube
Label or Dye Unconjugated
Product Line Ambion
5

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Purity or Quality Grade LD (Low DNA)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Standard
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT, SYBR
6

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads  DFS Item

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

7

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments  DFS Item

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
8

Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

9

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit  DFS Item
GREENER_CHOICE

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

Shipping Condition Room Temperature
Target DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Clean-Up Kit
Sample Type PCR Products
Isolation Technology ChargeSwitch™ Chemistry
For Use With (Application) Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line ChargeSwitch
10

Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)  DFS Item

The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.

11

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
12

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance High
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
13

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

14

Invitrogen™ PureLink™ PCR Purification Binding Buffer B2  DFS Item

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

15

MilliporeSigma™ Calbiochem™ EMPIGEN™ BB Detergent, 30% Solution

Can be used as detergent for solubilization of membrane components