Direct PCR Reagents and Kits
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Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
MilliporeSigma™ Calbiochem™ EMPIGEN™ BB Detergent, 30% Solution
Can be used as detergent for solubilization of membrane components
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
| Volume d’élution max | 20 μL |
|---|---|
| Technologie d’isolement | Silica Spin Column |
| Type de produit | PCR Micro Kit |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Cloning,Standard PCR |
| Contenu et stockage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Volume de matériau de démarrage | 125 μL |
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
| Volume d’élution min | 5 μL |
| Durée de fonctionnement | 10 min. |
| Format | Spin Column |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Plage de séparation | 100 bp to 12 kb |
| Gamme de produits | PureLink |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Compatibilité à haut débit | Yes |
|---|---|
| Méthode de détection | TaqMan |
| Format | Kit |
| Technologie d’isolement | Direct cell lysis |
| Cible | cDNA |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | RNA |
| Méthode PCR | 2-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| Conditions d’expédition | Frozen, Dry Ice |
| Type de kit | Direct Cell Lysis RT-qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
Applied Biosystems™ Sequencing Chemistry Installation Kit for 3500xL Series Genetic Analyzer
The 3500 platform can run a wide variety of applications
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
| Température de réaction optimale | 42°C |
|---|---|
| Gamme de produits | Ambion, Cells-to-cDNA |
| Type d’échantillon | Mammalian Cells |
| Type de produit final | cDNA |
| Taille (produit final) | 7 kb or less |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | M-MLV |
| Temps de purification | 2 hr. |
| À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
| Contenu et stockage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Standard |
| Type de produit | Gene Expression Cells-to-C Kit |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | Cell |
| Méthode PCR | 2-step RT-qPCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | DNA Polymerase |
| Format | Tube |
| Gamme de produits | Ambion, Cells-to-CT, TaqMan |
| Transcriptase inverse | M-MLV |
Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.
| Méthode de détection | TaqMan |
|---|---|
| Vitesse de réaction | 7 min. |
| Gamme de produits | Ambion, Cells-to-CT |
| Type d’échantillon | Mammalian Cells |
| Méthode PCR | 2-step RT-qPCR |
| Matériau de départ | 1-10 Cells |
| Conditions d’expédition | Dry Ice |
| Temps de purification | 7 min. |
| À utiliser avec (application) | RT-PCR |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
| GC-Rich PCR Performance | Medium |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Direct PCR Master Mix |
| Type d’échantillon | Tissue |
| En surplomb | Blunt |
| Méthode PCR | Direct PCR |
| Concentration | 2X |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Genotyping,Hot-start PCR |
| Comprend l’étiquette ou le colorant | Yes |
| Polymérase | Phire Hot Start II DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Direct PCR Master Mix |
| Hot Start | Built-In Hot Start |
| Type d’échantillon | Plant |
| En surplomb | Blunt |
| Méthode PCR | Direct PCR |
| Concentration | 2X |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Genotyping,Hot-start PCR |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ Template Generation System II Kit
Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
|---|---|
| Durée de fonctionnement | 5 min. |
| Format | Spin Column |
| Contenu et stockage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.