Real Time PCR Master Mixes

Real Time PCR Master Mixes
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Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUPâ„¢, SYBRâ„¢ |
Detection Method | SYBR |
Applied Biosystemsâ„¢ Power SYBRâ„¢ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBRâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,miRNA analysis,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 6 Pro, QuantStudioâ„¢ 7 Flex, QuantStudioâ„¢ 7 Pro, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal PCR Master Mix, no AmpEraseâ„¢ UNG
TaqManâ„¢ Universal PCR Master Mix, No AmpEraseâ„¢ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, GeneAmp 9600, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Multiplex Master Mix
Applied Biosystemsâ„¢ TaqManâ„¢ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
Polymerase | AmpliTaq Fast DNA Polymerase |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Product Type | Multiplex Master Mix |
Sample Type | DNA |
Reaction Speed | Fast |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | Multiplex qPCR |
Product Line | TaqManâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Universal Master Mix II, with UNG
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
GC-Rich PCR Performance | High |
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Content And Storage | Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Product Type | Universal Master Mix II |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, StepOneâ„¢, Standard Mode, StepOnePlusâ„¢, Standard Mode, ViiAâ„¢ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ PowerTrackâ„¢ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
Shipping Condition | Dry Ice |
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Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudioâ„¢ Absolute Q Digital PCR System, Bio-Rad CFX Connectâ„¢, Bio-Rad CFX384 Touchâ„¢, Bio-Rad CFX96 Touchâ„¢, BioRad CFX96â„¢, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiAâ„¢ 7 System, StepOneâ„¢ System, MJ Chromo4, MJ Opticon, StepOnePlusâ„¢ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | Applied Biosystemsâ„¢ |
Applied Biosystemsâ„¢ PowerUpâ„¢ SYBRâ„¢ Green & Enduraâ„¢ 384-well Plates Bundle
Bundle and save up to 50% off PowerUp SYBR Green Master Mix and Plates
Applied Biosystemsâ„¢ TaqPathâ„¢ BactoPureâ„¢ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
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Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiAâ„¢ 7 System, ViiA 7 Dx, QuantStudioâ„¢ Dx, StepOnePlusâ„¢ System, 7500 Fast Dx System, StepOneâ„¢ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Microbial Detection |
Product Line | TaqPathâ„¢ |
Applied Biosystemsâ„¢ Absolute Qâ„¢ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Applied Biosystemsâ„¢ Absolute Qâ„¢ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Applied Biosystemsâ„¢ VetMAXâ„¢ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Polymerase | AmpliTaqâ„¢ Fast DNA Polymerase |
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Activation Time | 5 min. |
Final Product Type | Mastermix |
Form | Liquid |
Reaction Speed | Fast |
Label or Dye | ROX |
Hot Start | Built-in Hot Start |
Concentration | 2X |
Starting Material | DNA/RNA |
Compatibility | TaqManâ„¢ |
For Use With (Application) | Animal Health |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix
Fisherbrandâ„¢ SYBRâ„¢ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |
Applied Biosystemsâ„¢ Absolute Qâ„¢ Universal DNA Digital PCR Master Mix (5X)
Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.
Applied Biosystemsâ„¢ TaqPathâ„¢ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.
Content And Storage | Store at 2°C to 8°C in the dark. |
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Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | Fast DNA Polymerase |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | QuantStudioâ„¢ Dx, 7500 Fast Dx, QuantStudioâ„¢ Absolute Q Digital PCR System |
Sample Type | cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqPathâ„¢ |