Antibiotics, Buffers and Supplements
Gibco™ Trypsin-EDTA (0.25%), phenol red
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
Gibco™ Trypan Blue Solution, 0.4%
Routinely used as a cell stain to assess cell viability using the dye exclusion test
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
Used as a growth supplement for cell culture medium, to increase cell growth and viability
Gibco™ Bacto™ Yeast Extract
Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.
Gibco™ GlutaMAX™ Supplement
An alternative to L-glutamine, with increased stability that improves cell health
Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
Unlike porcine trypsin, free of animal-derived components
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ Gurr Buffer Tablets
A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain
Gibco™ Trypsin-EDTA (0.5%), no phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
Gibco™ HT Supplement (100X)
HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.
MES (Fine White Crystals), Fisher BioReagents
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid, 2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals
Corning™ Cell Culture Phosphate Buffered Saline (1X)
With low endotoxin content. Without calcium and magnesium.
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O
MilliporeSigma™ Polybrene Infection/Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
Gibco™ HAT Supplement (50X)
HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells
Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
MilliporeSigma™ Alizarin-Red Staining Solution
a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).