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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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2

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Type de produit Cell Culture Dissociation Reagent
Stérilité Sterile
pH 7 to 7.6
Sans additif No Calcium,No Magnesium,No Sodium Bicarbonate
Forme Liquid
Contenu et stockage -20°C
3

Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

4

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Type de produit Cell Culture Dissociation Reagent
Testé pour In vitro Bioassay
Concentration 1 X
Source Animal Origin
Conditions d’expédition Wet Ice
Avec additifs Phenol Red
Forme Liquid
Contenu et stockage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Sterilization Method Sterile-filtered
Stérilité Sterile-filtered
Classification Animal Origin
Gamme de produits Gibco
Type de cellule Mammalian Cells
Chélateurs No EDTA
Osmolalité 280 - 310 mOsm/kg
Durée de conservation 24 Months
5

Gibco™ Soybean Trypsin Inhibitor, powder  DFS Item

Trypsin inhibited at a molar ratio of 1:1 and chymotrypsin and plasmin to lesser extent

6

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Type de produit Collagenase
Stérilité Non-sterile
Durée de conservation 24 Months
Conditions d’expédition Room Temperature
Forme Lyophilized
Contenu et stockage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
7

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

8

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium

Used to enzymatically release adherent cells from tissue culture plates for passaging.

9

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

10

Gibco™ Defined Trypsin Inhibitor  DFS Item
GREENER_CHOICE

Animal origin-free

11

Gibco™ Trypsin/EDTA Solution (TE)  DFS Item
GREENER_CHOICE

With EDTA and phenol red

12

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Type de produit Cell Culture Dissociation Reagent
Stérilité Sterile
Concentration 1 X
Formule Chelating and stabilizing agents
Durée de conservation 2 Years
À utiliser avec (application) Cell Dissociation
Forme Liquid
Contenu et stockage Store at room temperature or at 4°C.
13

Gibco™ Cell Dissociation Buffer, enzyme-free, PBS  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

14

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

15

MilliporeSigma™ PluriSTEM™ Dispase-II Solution

a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.