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NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
Content And Storage | This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase. Store the product at 4°C. |
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Form | Liquid |
Readout | End Point |
Compatible Cells | Mammalian Cells |
Target Enzyme | Alkaline Phosphatase |
Substrate Type | AP (Alkaline Phosphatase) Substrate |
For Use With (Application) | Enzyme Assay |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Chemiluminescence |
Substrate | CSPD™ |
Assay | Reporter Assay |
Substrate Properties | Chemical Substrate |
For Use With (Equipment) | Luminometer (Microplate) |
Product Type | Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System |
Sample Type | Cell Cultures |
Label or Dye | Unconjugated |
Product Line | NovaBright™, Phospha-Light™ |
λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen
A CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker
Designed to purify double-stranded DNA (dsDNA) generated by enzymatic reactions such as cDNA synthesis and PCR
A rapid and robust treatment for RNA derived from whole blood that uses a novel, non-enzymatic technology to rapidly deplete >95% of the alpha and beta globin mRNA from total RNA preparations.
Amplifies as little as 100pg of input RNA for GeneChip™ analysis
Format | Kit |
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Reverse Transcriptase | ArrayScript™ |
Includes Label or Dye | No |
Product Type | aRNA Amplification Kit |
Sample Type | Poly(A+) RNA, Total RNA |
Product Line | Ambion™, MessageAmp™ |
Add to the RNA sample for use as a template for preparing labeled cDNA
For the measurement of miRNA expression in cells
For the fragmentation of aRNA prior to hybridization with oligonucleotide arrays
For use in reverse transcription reactions to append a T7 RNA polymerase promoter to cDNA fragments
Substrates cleaved within cell for fluorescent product
Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript
Reaction produces blue fluorescent product
Provides reagents for aRNA amplification with Amino allyl NTP incorporation