Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells

Gal-Screen homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
• The kinetics of the glow-assay provides a window during which measurements may be performed, facilitating HTS applications where assay automation is used
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
• Assay sensitivity is 100 to 1000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
• Assay can be completed in about one hour, providing fast assay turnaround

Specifications

• Compatible Cells: Mammalian Cells, Yeast Cells
• Detection Method: Chemiluminescent
• For Use With (Equipment): Luminometer (Microplate)
• Form: Liquid
• Format: 96-well plate
• High Throughput Compatibility: High Throughput-Compatible
• Readout: End Point
• Substrate: Galacton-Star
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase