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Invitrogen™ Random Primers DNA Labeling System

Catalog No. 18187013
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30 Reactions
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Catalog No. Quantity
18187013 30 Reactions
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Catalog No. 18187013 Supplier Invitrogen™ Supplier No. 18187013
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Description

Includes

  • DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM
  • MgCl2 , 500mM NaCl], vial of dilution buffer

Ideal for radioactively labeling DNA, particularly fragments under 1 kb

  • Yields >109 cpm/μg control DNA using [ α-32 P]-dCTP
  • 25ng of DNA in one reaction
  • Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a random primers labeling reaction

DNA and RNA Purification and Analysis, DNA Labeling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Nucleic Acid Labeling and Oligo Synthesis, Southern Blotting

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

Product Type DNA Labeling System
Detection Method Radioactive
Content And Storage Components of the Random Primers DNA Labeling System are listed in the table to the right. Store at -20°C.
Final Product Type Probes (Labeled DNA)
Label or Dye 32P (Phosphorous-32)
Includes Label or Dye No
Labeling Target DNA (General)
Labeling Method Direct Labeling
Shipping Condition Approved for shipment on Wet or Dry Ice
Quantity 30 Reactions

Frequently Asked Questions (FAQs)

How can I label COT-1 DNA?

Probes can be labeled with 32P by random primer or nick translation procedures using the Random Primers DNA Labeling System (Cat. No.18187-013) or Nick Translation System (Cat. No. 18160-010). Biotinylated COT-1 DNA can be prepared by nick translation with the BioNick Labeling System (Cat. No. 18247-015) or by the BioPrime DNA Labeling System (Cat. No. 18094-011). Improved results can be obtained when the COT-1 DNA is first ligated to itself to provide an optimum template.

For Research Use Only. Not for use in diagnostic procedures.