Gene Reporter Systems
Gene Reporter Systems
Promega Luciferase Reporter Assays to Study Nuclear Receptors: pFN26A (BIND) hRluc-neo Flexi™ Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
Invitrogen™ Mouse Cot-1 DNA
Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members
Invitrogen™ Human Cot-1 DNA™
Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids
Invitrogen™ Human Cot-1 DNA™-Fluorometric QC
Quantifying by fluorometry provides greater data accuracy
Invitrogen™ Yeast tRNA
Supplied in lyophilized form
Invitrogen™ λ DNA (cIind 1 ts857 Sam 7)
λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen
Invitrogen™ Biotin-14-CTP
A CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker
Invitrogen™ Biotin-14-dCTP
Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling
MilliporeSigma™ Calbiochem™ Coelenterazine
A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.
Invitrogen™ BLOCK-iT™ Pol II miR RNAi Expression Vector Kit with EmGFP
Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript
Invitrogen™ BLOCK-iT™ Adenoviral RNAi Expression System
Used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Invitrogen™ BLOCK-iT™ Inducible Pol II miR RNAi Expression Vector Kit with EmGFP
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript.
Invitrogen™ BLOCK-iT™ U6 RNAi Entry Vector Kit
Provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi)