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NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.
Content And Storage | This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase. Store the product at 4°C. |
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Form | Liquid |
Readout | End Point |
Compatible Cells | Mammalian Cells |
Target Enzyme | Alkaline Phosphatase |
Substrate Type | AP (Alkaline Phosphatase) Substrate |
For Use With (Application) | Enzyme Assay |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Chemiluminescence |
Substrate | CSPD™ |
Assay | Reporter Assay |
Substrate Properties | Chemical Substrate |
For Use With (Equipment) | Luminometer (Microplate) |
Product Type | Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System |
Sample Type | Cell Cultures |
Label or Dye | Unconjugated |
Product Line | NovaBright™, Phospha-Light™ |
Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members
Effective library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids
Quantifying by fluorometry provides greater data accuracy
Shipping Condition | Approved for shipment at Room Temperature or on Wet or Dry Ice |
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Content And Storage | Store in freezer (-5 to -30°C). |
Form | Lyophilized Powder |
Product Type | Yeast tRNA |
Reagent Type | Yeast tRNA |
For Use With (Application) | Carrier for Nucleic Acid Precipitation |
λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen
A CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker
Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling
A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.
Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript
Used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Format | Kit |
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Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Bacterial or Yeast Strain | TOP10 |
Vector | TOPO-TA Cloning Vectors |
Product Line | One Shot™ |
The BLOCK-iT™ Pol II miR RNAi Expression Vector Kits combine the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript.
Provides a simple, streamlined approach to cloning short hairpin RNA (shRNA) sequences for testing in transient transfections for RNA interference (RNAi)