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Protein Modification Reagents

Chemical agents used to modify the amino acid side chains of proteins in order to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.
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1

Invitrogen™ Measure-IT™ Thiol Assay Kit  DFS Item

Provides easy and accurate quantitation of thiol

4

Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

5

MilliporeSigma™ Calbiochem™ Cleland’s Reagent

Protective agent for SH groups

Content And Storage 2°C to 8°C
CAS 12-3-3483
Product Type Calbiochem Cleland’s Reagent
Form Powder
Molecular Weight (g/mol) 154.24
Solubility EtOH, Water
Purity ≥97% by titration
Synonym DTT, Dithiothreitol
Molecular Formula C4H10O2S2
6

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

7

MilliporeSigma™ Calbiochem™ BAPTA/AM

Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA

Membrane Permeable Y
Content And Storage -20°C
CAS 126150-97-8
Product Type Calbiochem BAPTA/AM
Form Solid
Solubility DMSO
Purity ≥90% by TLC
Synonym 1,2-bis(o-Aminophenoxy)ethane-N,N,Nʹ,Nʹ-tetraacetic Acid Tetra(acetoxymethyl) Ester
8

MilliporeSigma™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

10

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

11

MilliporeSigma™ Calbiochem™ TPEN

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes

14

MilliporeSigma™ Calbiochem™ Cleland’s Reagent, ULTROL™ Grade

Protective agent for SH groups

Content And Storage 2°C to 8°C
CAS 12-3-3483
Product Type Calbiochem Cleland’s Reagent
Form Solid
Molecular Weight (g/mol) 154.24
Solubility EtOH, Water
Purity ≥99% by titration
Synonym Dithiothreitol, DTT
Molecular Formula C4H10O2S2
15

Thermo Scientific™ Staphylococcus aureus V-8 Protease

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.