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Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Catalog No. PI23460
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PI23460 1 Reaction
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Catalog No. PI23460 Supplier Thermo Scientific™ Supplier No. LSG23460

Description

Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.

Thermo Scientific™ Pierce Sulfhydryl Addition Kit is a complete set of reagents, including SATA, to enable modification of primary amines to free sulfhydryl groups for use in heterobifunctional crosslinking techniques.

Highlights:

  • Reacts with primary amines to add protected sulfhydryls
  • An easy deprotection step generates a free sulfhydryl group
  • Thermo Scientific™ Sulfhydryl Addition Kit contains all necessary components for thiolation, deprotection, purification and quantitation

Specifications

Chemical Reactivity Amine
Content And Storage Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein
• SATA, 2 mg
• Hydroxylamine·HCl, 5 mg
• Conjugation Buffer Stock (10X), 20 mL
• PBS pack (makes 500 mL), 1 pack
• Dimethylformamide (DMF), 1 mL
• Dextran Desalting Column, 1 x 5 mL
• Column Extender, 1
• Ellman's Reagent (DTNB), 2 mg
• Cysteine·HCl, 20 mg

Store at 4°C.
Label or Dye Sulfhydryl Group
Label Type Conjugating Groups
Product Line Pierce
Crosslinker Type Heterobifunctional
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Spacer Short (<10 Å)
Form Powder
Reagent Type SATA
Quantity 1 Reaction
Solubility DMF, DMSO
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.