Protein Modification Reagents
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent
A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.
Thermo Scientific™ MM(PEG)24 Methyl-PEG-Maleimide Reagent
Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).
Thermo Scientific™ Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)
Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
MilliporeSigma™ ALCALASE™ Enzyme, Bacillus licheniformis
Suitable for hydrolysis of proteins
Thermo Scientific™ CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ Pierce™ Cysteine-HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).
Gibco™ PNGase F Glycan Cleavage Kit
PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.
Thermo Scientific™ Pierce™ Immobilized Reductant Column, 2 mL
Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction.
MilliporeSigma™ Calbiochem™ N-Glycolylneuraminic Acid
Formed by action of CMP-Neu5Ac hydroxylase.
Invitrogen™ Protease QF
Protease QF, 180 μL, is an enzyme in aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).