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Invitrogen™ Dithiothreitol (DTT)

Catalog No. D1532
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D1532 1 g
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Catalog No. D1532 Supplier Invitrogen™ Supplier No. D1532
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Description

Dithiothreitol (DTT)

Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by dithiothreitol (DTT).

Specifications

Content And Storage Store at room temperature.
Shipping Condition Room Temperature
Form Solid
Quantity 1 g
Product Type Dithiothreitol

Frequently Asked Questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%
How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

For Research Use Only. Not for use in diagnostic procedures.