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Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Catalog No. T2556
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T2556 1 g
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Catalog No. T2556 Supplier Invitrogen™ Supplier No. T2556
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Description

Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by TCEP (tris-(2-carboxyethyl)phosphine). Unlike DTT (dithiothreitol), TCEP does not contain thiols and therefore usually does not need to be removed prior to thiol modification.

Specifications

Content And Storage Store at room temperature.
Shipping Condition Room Temperature
Form Solid
Quantity 1 g

Frequently Asked Questions (FAQs)

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

For Research Use Only. Not for use in diagnostic procedures.