Crosslinking Reagents
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))
Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.
Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.
Thermo Scientific™ L-Photo-Leucine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ EMCH (N-ε-maleimidocaproic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 10-atom (11.6A) spacer arm.
Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
MilliporeSigma™ Calbiochem™ SATA
Reacts with primary amines to add protected sulfhydryl groups.
Thermo Scientific™ GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ BMH (bismaleimidohexane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.
Thermo Scientific™ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.