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Crosslinking Reagents

Reagents containing reactive end groups that respond to the presence of specific functional groups by forming bonds between polymer chains. Ideal for various applications including conjugation reactions, biomolecule labeling, and molecular purification.
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1

Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.

2

Thermo Scientific™ Sulfo-MBS (m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester)

Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.

3

Invitrogen™ Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)  DFS Item

The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols.

4

MilliporeSigma™ Calbiochem™ SATA

Reacts with primary amines to add protected sulfhydryl groups.

7

Thermo Scientific™ SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 10-atom (11.6A) spacer arm.

8

Thermo Scientific™ DTME (dithiobismaleimidoethane)

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.

9

Thermo Scientific™ BMH (bismaleimidohexane)

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.

10

Thermo Scientific™ SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom (10.6A) aminobenzoate spacer arm.

11

Thermo Scientific™ Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.

12

Thermo Scientific™ Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)

Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.

13

Thermo Scientific™ LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups at ends of 10-atom (15.7A) spacer arm.

14

Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

15

Thermo Scientific™ BS2G-d0 (bis(sulfosuccinimidyl) glutarate-d0)

Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.