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Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers


Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Manufacturer: thermo scientific™  26167

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Catalog No. PI26167

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Description & Specifications

Specifications

Spacer Arm Length 3.9Å
Formulation Succinimidyl 4,4'-azipentanoate; solid
Molecular Weight 225.2
Water Soluble No
Description Pierce SDA
PEGylated No
Chemical Reactivity Amine-to-Nonselective
Membrane Permeable Yes
Sufficient For 222mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
Quantity 50mg
Cleavable No

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light