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  6. Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)

Catalog No. PIPG82086
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PIPG82085 100 mg
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Catalog No. PIPG82086 Supplier Thermo Scientific™ Supplier No. PG82086

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.

Thermo Scientific Pierce Premium Grade Sulfo-SMCC is our highest quality formulation of amine-to-sulfhydryl crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade Sulfo-SMCC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-SMCC is a water-soluble, non-cleavable and membrane-impermeable amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms). NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to nearly 10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.

Features of Sulfo-SMCC:

  • Sulfo-SMCC—a water-soluble amine-to-sulfhydryl crosslinker with a stabilizing cyclohexane spacer arm
  • High quality—identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity—enhanced level of testing and characterization compared to standard grade
  • Lot retention—ample supply of past lots retained to ensure future process testing
  • Change management—Change Control Notification (CCN) service
  • Consistent manufacture—batch-specific manufacturing documentation review

Applications:

  • Enzyme labelling of antibodies - both enzyme and antibody specificity can be preserved
  • Create specific bioconjugates via one- or two-step crosslinking reactions
  • Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Products

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) (Cat. No. 22322)

Specifications

Chemical Reactivity Amine-Sulfhydryl
Cleavable No
Molecular Weight (g/mol) 436.37
PEGylated No
Spacer Arm Length 8.3 Å
Content And Storage Upon receipt store desiccated at -20°C.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Maleimide, Sulfo-NHS Ester
Spacer Short (<10 Å)
Form Powder
Quantity 1 g
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)?

Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Is the cyclohexane ring in Sulfo-SMCC crosslinker cis or trans?

The cyclohexane ring in Sulfo-SMCC crosslinker is trans.
Can Sulfo-SMCC crosslinker reagent be reconstituted in an organic solvent (DMF or DMSO) and added to an aqueous reaction mixture?

Yes, Sulfo-SMCC can be reconstituted in an organic solvent (DMF or DMSO) and added to an aqueous reaction mixture.
Is Sulfo-SMCC crosslinking reagent water soluble?

Yes, Sulfo-SMCC is water soluble.
Can the Sulfo-SMCC cross-linking agent be added directly to the reaction mixture?

Yes, Sulfo-SMCC can be added directly to the reaction mixture.
Can Sulfo-SMCC be cleaved?

No, Sulfo-SMCC cannot be cleaved.
Will the Sulfo-SMCC cross-linker penetrate a cell membrane?

No, the Sulfo-SMCC cross-linker cannot penetrate a cell membrane.
Is there a method to detect if a protein contains accessible sulfhydryl groups?

Yes, Ellman's reagent (Cat. No. 22582) can be utilized to determine if a protein contains free sulfhydryl groups.
When crosslinking using Sulfo-SMCC, can proteins be modified to add free sulthydryls?

Yes, Trauts Reagent (Cat. No. 26101) will react with primary amine groups to generate a free sulfhydryl group. SATA (Cat No. 26102) will react with primary amine groups to generate a protected free sulfhydryl group that can be released prior to conjugation.
Will the maleimide chemistry in Sulfo-SMCC crosslinker react with disulfides?

No, the disulfides must first be reduced to free sulfhydryls utilizing a reducing agent. If the reducing agent contains free -SH (DTT, B-ME etc.) it must first be removed by desalting. Dialysis may not be used as a substitute for desalting, since over time, disulfide linkages will re-form. 10-50 mM EDTA in the reaction buffer can help slow the re-oxidation of sulfhydryls to disulfides.

How is the Sulfo-SMCC crosslinking reaction quenched?

1M Tris (or glycine), pH 7.5 can be added to the reaction mixture to a final concentration of approximately 20-50 mM to quench the NHS-Ester, or the product may be desalted to remove un-reacted cross-linker. Freshly prepared cysteine may be used to quench the maleimide chemistry.
Can I make up a Sulfo-SMCC stock solution and store the cross-linker long-term?

Long-term storage is not recommended since the NHS-ester group is susceptible to hydrolysis. Hydrolysis begins upon reconstitution. Even organic solvents such as DMSO and DMF are hygroscopic, as a result, they tend to absorb water, which in turn also promotes the hydrolysis of the NHS-Ester.
What does "store under nitrogen" mean for Sulfo-SMCC crosslinker?

A gentle stream of nitrogen gas is held over the opening of the vial to flush the oxygen from the vial. The cap is then immediately placed on the vial. If storing under nitrogen, caution should be taken to ensure that the stream of nitrogen is gentle enough, so that the powdered product will not be blown out of the bottle. If nitrogen is not available, weigh the material as quickly as possible and immediately cap. Store desiccated.
What is the recommended storage condition for Sulfo-SMCC crosslinker?

The product ships under ambient temperature conditions. Upon receipt, the product should be stored at -20 degrees C in a dessicator or with a desiccant bag, and the vial should be warmed to room temperature before opening.
What is the expiration date for the Sulfo-SMCC crosslinker?

This product has no set expiration date. We monitor the bulk material in such a manner that allows us to warranty the product for one year from the date of shipment. It may still be usable beyond the one year if handled properly. However, using material older than one year is typically not recommended since the material easily hydrolyzes upon exposure to moisture.
How much Sulfo-SMCC cross-linking agent should I use?

A 10-20 fold molar excess of cross-linker to protein is recommended for protein solutions greater than 5 mg/mL. A 20-100 fold molar excess of cross-linker to protein is recommended for protein solutions less than 5 mg/mL.
What is the recommended concentration of the Sulfo-SMCC crosslinker stock solution?

An initial stock concentration of 5-12 mM (2 - 5 mg/mL) is recommended, or the cross-linking reagent may be added directly to the reaction mixture.
What buffers are not recommended for protein crosslinking using Sulfo-SMCC?

Buffers containing primary amines, primary sulfhydryls, Tris, ethanolamine, glycine, cysteine or lysine should not be used, since they will quench the reaction. Imidazole, glutathione, or sulfhydryl containing reducing reagents are also not recommended as buffer components.
What is the recommended sample buffer and reconstitution buffer to obtain optimal protein cross-linking using Sulfo-SMCC?

We recommend using 100 mM sodium phosphate, 0.15 M NaCl, pH 7.2 (PBS). HEPES, bicarbonate/carbonate, and borate buffers may be substituted for PBS. Other buffers may also be substituted provided they do not contain primary amines or primary sulfhydryls. Recommended pH is 7-9. The reaction can be performed over a pH range of 7-9, however as the pH increases, the rate of hydrolysis of the NHS-Ester also increases as well as the likelihood that the maleimide will react with amines. Once the cross-linker is reconstituted, it must be used immediately.

For Research Use Only. Not for use in diagnostic procedures.