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Thermo Scientific™ Pierce™ NHS-Phosphine Staudinger Ligation Reagents

Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.

Manufacturer:  Thermo Scientific™ 88900

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Catalog No. PI88900




Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine groups (PPh2) react with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, these NHS-Phosphine reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.


  • Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents

  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

Description & Specifications


Pierce NHS-Phosphine
Phosphine functionalized as N-hydroxysuccinimide ester; powder solid
22mL of typical 1X (1mM) reaction solution, or approx. 5 uses at 2mg reagent per use