Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

• The highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount
• Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support
• A water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation
• In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters
• Store dessicated at -20°C

Features of Premium Grade EDC

• High quality – identity and purity confirmed by several tests
• Product integrity – enhanced level of testing and characterization compared to standard grade
• Lot retention – ample supply of past lots retained to ensure future process testing
• Change management – Change Control Notification (CCN) service
• Consistent manufacture – batch-specific manufacturing documentation review

Applications

• Conjugate carboxyl and amino groups among peptides and proteins
• Couple haptens to immunogenic carrier proteins
• Immobilize peptide antigens to affinity-purify antibodies
• Create NHS-activated, amine-reactive labeling compounds
• Crosslink proteins to carboxyl coated beads or surfaces
• Activate nanoparticles with amine-reactive sulfo-NHS esters