Crosslinking Reagents
Crosslinking Reagents
Thermo Scientificâ„¢ Pierceâ„¢ EDC, No-Weighâ„¢ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientificâ„¢ Mass Spectrometry Compatible Crosslinkers
Improve mass spectrometry detection of crosslinked proteins and interactions with DSPP and TBDSPP heterotrifunctional crosslinkers.
Thermo Scientificâ„¢ NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific Pierceâ„¢ Pierceâ„¢ SM(PEG)2, No-Weighâ„¢ Format
Thermo Scientificâ„¢ Pierceâ„¢ SM(PEG)2 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientificâ„¢ Mass Spectrometry Cleavable Crosslinkers
Improve sensitivity of mass spectrometry detection of crosslinked proteins with MS-cleavable DSSO and DSBU heterotrifunctional crosslinkers.
Thermo Scientificâ„¢ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientificâ„¢ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weighâ„¢ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientificâ„¢ DSS (disuccinimidyl suberate), No-Weighâ„¢ Format
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.
Thermo Scientificâ„¢ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weighâ„¢ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientificâ„¢ DST (disuccinimidyl tartrate)
Crosslink amines to amines with disuccinimidyl tartrate, composed of NHS-ester groups and a diol-containing spacer arm (6.4A) that is periodate-cleavable.
Thermo Scientificâ„¢ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))
Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.
Thermo Scientificâ„¢ BS3-d0 (bis(sulfosuccinimidyl) suberate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientificâ„¢ SM(PEG)6 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).