UserName
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Description | EDC |
---|---|
Méthode d’étiquetage | Chemical Labeling |
Perméabilité cellulaire | No |
Cleavable | No |
Type de produit | Crosslinker |
Longueur du bras espaceur | 0.0 Å |
Réactivité chimique | Amine-Carboxyl |
Conditions d’expédition | Wet Ice |
Forme | Powder |
Contenu et stockage | Upon receipt store desiccated at -20°C. |
Pégylé | No |
Entretoise | Short (<10 Å) |
Poids moléculaire | 191.7 |
Format | Standard, Single-use, Premium-grade |
Type d’agent de réticulation | Heterobifunctional |
Solubilité | Water |
Hydrosoluble | Yes |
Groupement de réactifs | Carbodiimide |
Used to label E.coli thioredoxin.
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups at ends of 10-atom (15.7A) spacer arm.
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 4-atom (4.4A) spacer arm.
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 8-atom (9.4A) spacer arm.
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.
Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.
Chemically conjugate any two purified proteins (antibodies, enzymes, carriers, etc.) using sulfo-SMCC crosslinker, a complete kit with modification reagents and buffers.
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).