Miscellaneous Molecular Biology Reagents
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Pharmalytes™ pH range 4.0 - 6.5, MilliporeSigma™ Supelco™
Pharmalytes™ are ampholytes which are used to create a pH gradient under application of an electric field.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Thermo Scientific™ Wash Solution I (concentrated)
Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (Cat. No. K0491, K0492) and may be purchased separately.
Thermo Scientific™ EDTA
Chelate alkaline earth metals. Thermo Scientific™ Pierce EDTA is highly purified for use in a variety of laboratory methods.
Invitrogen™ Formamide (Deionized)
Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon.
Invitrogen™ dNTP Mix (2.5 mM)
The same high-quality deoxynucleotide triphosphate solutions provided in the Invitrogen™ RETROscript™ First Strand cDNA Synthesis Kit for RT-PCR.
Invitrogen™ Guanidine Thiocyanate
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents.
Invitrogen™ SSC (20X), RNase-free
Molecular biology grade, Invitrogen 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ SDS, 10% Solution, RNase-free
Molecular biology grade, Invitrogen SDS, 10% Solution is supplied in one bottle containing 500 mL.
MilliporeSigma™ Calbiochem™ 5-Bromo-2'-deoxyuridine
Can be used both in vitro and in vivo to label DNA during DNA synthesis where it is incorporated into DNA in place of thymidine