Miscellaneous Molecular Biology Reagents
MilliporeSigma™ Novagen™ Pellet Paint™ Co-Precipitant
Compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
MilliporeSigma™ Novagen™ X-Gal Solution
Convenient for blue/white screening
Thermo Scientific™ Phenol:Chloroform + Tris Buffer
Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Thermo Scientific™ Sodium Acetate Solution (3 M), pH 5.2
Precipitate DNA and RNA with this 0.22μm membrane-filtered, sterile, nuclease-free solution of sodium acetate.
Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free
Invitrogen Molecular biology grade, 3M Potassium Acetate, pH 5.5 solution is supplied in one bottle containing 100 mL.
Invitrogen™ KCl (2 M), RNase-free
Invitrogen molecular biology grade, 2M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)
In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
MilliporeSigma™ Calbiochem™ D(+)Melezitose, Monohydrate
Substrate for α-glucosidase
MilliporeSigma™ Calbiochem™ Polyvinylpyrrolidone
Polymer used as a pharmaceutical aid, complexing agent and solubilizer
MilliporeSigma™ Calbiochem™ 2-Deoxy-D-glucose
Reported to reduce the incidence of doxorubicin-induced apoptosis
MilliporeSigma™ Calbiochem™ D(+)Galactosamine, Hydrochloride
Powerful inhibitor of hepatic RNA synthesis
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.
MilliporeSigma™ Calbiochem™ Deoxyribonucleic Acid, Sodium Salt, Salmon Testes
Purified DNA from salmon testes