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Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

EDGE
Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
2

Invitrogen™ Anza™ T4 DNA Ligase Master Mix  DFS Item

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Shipping Condition Approved for shipment on Wet or Dry Ice
Product Type T4 DNA Ligase Master Mix
Enzyme T4 DNA Ligase
Compatible Buffer Elution Buffer, 1X Anza Buffer
Concentration 4X
Product Line Anza
5

Thermo Scientific™ Uracil-DNA Glycosylase, heat-labile

Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone.

6

Invitrogen™ Topoisomerase I  DFS Item

Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds

7

Invitrogen™ M13KO7 Helper Phage  DFS Item

Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication

8

Thermo Scientific™ RNase T1 (1000 U/μL)

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Product Type RNase T1
Enzyme RNase
Concentration 1000 U/μL
9

MilliporeSigma™ Calbiochem™ MACERASE™ Pectinase, Rhizopus sp.

Useful in isolating intact cells and protoplasts from higher plants

Content And Storage 2°C to 8°C
Form Solid
Product Type Pectinase
Color Pale Amber
Source Rhizopus sp.
10

Invitrogen™ Ambion™ DNase I (RNase-free)  DFS Item

Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin

Shipping Condition Dry Ice
Content And Storage -20°C
Product Type DNase I
Enzyme DNase
Product Line Ambion
11

MilliporeSigma™ Calbiochem™ β1-2,3,4,6-N-Acetylglucosaminidase

Catalyzes hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates

13

Invitrogen™ Ambion™ RNase H, from E. coli, 10 U/μL  DFS Item

Endonuclease-free, exonuclease-free, protease-free

Shipping Condition Dry Ice
Content And Storage -20°C
Enzyme RNase
Concentration 10 U/μL
Product Line Ambion
14

Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)  DFS Item

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Sensitive to Heat Inactivation Yes
Form Liquid
Product Type HiFi Cas9
Purity ≥90%
Concentration 5 μg/μL
Source E. coli
15

Thermo Scientific™ Klenow Fragment, LC (2 U/μL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.