Modifying Enzymes
Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
MilliporeSigma™ Novagen™ Chicken Egg White Lysozyme Solution
For purification of both DNA, protein, and Gram-positive bacterial lysis
MilliporeSigma™ Calbiochem™ Horseradish Peroxidase
Suitable for conjugation to proteins and antibodies and as cytochemical marker in immunohistochemical staining
MilliporeSigma™ Novagen™ KOD Xtreme™ Hot Start DNA Polymerase
For challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates
MilliporeSigma™ Novagen™ rLysozyme™ Solution
For lysis of Gram-negative bacteria
MilliporeSigma™ Novagen™ KOD Hot Start DNA Polymerase
For PCR amplification of long strand and GC-rich DNA templates, cloning and cDNA amplification applications
MilliporeSigma™ Calbiochem™ Highly Purified Protease-Free RNase A, Bovine Pancreas
For selective degradation of RNA
MilliporeSigma™ Calbiochem™ RNase A, Bovine Pancreas
For selective degradation of RNA
Invitrogen™ T4 DNA Ligase Buffer
T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
Thermo Scientific™ Lambda Exonuclease (10 U/μL)
Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Invitrogen™ Anza™ Alkaline Phosphatase
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Invitrogen™ S1 Nuclease
Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides