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Invitrogen™ T4 DNA Ligase (5 U/μL)

Catalog No. 15224041
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250 U
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Catalog No. Quantity
15224041 250 U
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Catalog No. 15224041 Supplier Invitrogen™ Supplier No. 15224041
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Description

Includes

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000].

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini

The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.

Unit Definition: One unit catalyzes the exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)

Unit Reaction Conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2 , 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C.

Order Info

Shipping Condition: Dry Ice

Specifications

Concentration 5 U/μL
Content And Storage T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
Shipping Condition Dry Ice
Enzyme Ligase
Compatible Buffer 5X Reaction Buffer
Quantity 250 U
Product Type T4 DNA Ligase

For Research Use Only. Not for use in diagnostic procedures.