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Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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1 – 15 of 245 results
1

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

EDGE
Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
2

Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)  DFS Item

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Sensitive to Heat Inactivation Yes
Form Liquid
Product Type HiFi Cas9
Purity ≥90%
Concentration 5 μg/μL
Source E. coli
3

MilliporeSigma™ Calbiochem™ MACERASE™ Pectinase, Rhizopus sp.

Useful in isolating intact cells and protoplasts from higher plants

Content And Storage 2°C to 8°C
Form Solid
Product Type Pectinase
Color Pale Amber
Source Rhizopus sp.
4

Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Product Type T4 DNA Ligase
Enzyme DNA Ligase
Compatible Buffer 10X T4 DNA Ligase Buffer
Concentration 5 U/μL
7

Thermo Scientific™ Micrococcal Nuclease (300 U/μL)

Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.

8

MilliporeSigma™ Calbiochem™ Y-27632

Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.

Content And Storage Hygroscopic
Protect from Light
Form Solid
Molecular Weight (g/mol) 338.3g/mol
Color White
Purity 95% by HPLC
9

Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix  DFS Item

Catalyzes in vitro recombination of PCR products to generate entry clones

Shipping Condition Dry Ice
Content And Storage Gateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 μg/μl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type BP Clonase Enzyme Mix
Enzyme BP Clonase
Compatible Buffer Enzyme Buffer
Product Line Clonase, Gateway
11

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL  DFS Item

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

13

Invitrogen™ S1 Nuclease  DFS Item

Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides

14

Invitrogen™ T4 DNA Ligase (5 U/μL)  DFS Item

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini

15

Invitrogen™ Topoisomerase I  DFS Item

Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds