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Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)

Catalog No. 4403083
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Catalog No. 4403083 Supplier Applied Biosystems™ Supplier No. 4403083
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Description

Includes

Reagents sufficient for 4,000 sample preparation reactions at 50μL/ reaction and 4,000 PCR reactions at 5μL for each reaction.

Go from Biological Sample to Genotyping Results in less than one hour.

The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

  • DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
  • The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
  • Compatible with both Fast and Standard mode thermal cycling conditions
  • Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
  • Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
  • Extended PCR cycling for poorly amplified or sample-limited reactions

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage Contains Lysis Reagents (2° to 8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C): 10 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
GC-Rich PCR Performance Low
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
Technique SNP Genotyping (Real-Time PCR-Based)
For Use With (Equipment) 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Fast Mode, StepOne™ Standard Mode
Genotyping Target SNPs (Unknown or Numerous), SNPs (Known)
Passive Reference Dye ROX (Pre-mixed)
Product Line Sample-to-SNP, TaqMan
Product Type Genotyping Reagents
Quantity 1 kit
Sufficient For 400 Reactions
Hot Start Built-In Hot Start
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Volume 20 mL
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Frequently Asked Questions (FAQs)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".

For Research Use Only. Not for use in diagnostic procedures.