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Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)

Catalog No. 4403083
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Catalog No. 4403083 Supplier Applied Biosystems™ Supplier No. 4403083
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Description

Includes

Reagents sufficient for 4,000 sample preparation reactions at 50μL/ reaction and 4,000 PCR reactions at 5μL for each reaction.

Go from Biological Sample to Genotyping Results in less than one hour.

The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

  • DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
  • The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
  • Compatible with both Fast and Standard mode thermal cycling conditions
  • Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
  • Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
  • Extended PCR cycling for poorly amplified or sample-limited reactions

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage Contains Lysis Reagents (2° to 8°C): 20 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C): 10 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
GC-Rich PCR Performance Low
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
Technique SNP Genotyping (Real-Time PCR-Based)
For Use With (Equipment) 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Fast Mode, StepOne™ Standard Mode
Genotyping Target SNPs (Unknown or Numerous), SNPs (Known)
Passive Reference Dye ROX (Pre-mixed)
Product Line Sample-to-SNP, TaqMan
Product Type Genotyping Reagents
Quantity 1 kit
Sufficient For 400 Reactions
Hot Start Built-In Hot Start
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Volume 20 mL
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For Research Use Only. Not for use in diagnostic procedures.