Direct PCR Reagents and Kits
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Résultats de la recherche filtrée
Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| Type de réactif | Cell Lysis |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | 7 min. |
| Format | Tube |
| Type de produit | 1-Step qPCR kit |
| Gamme de produits | Cells-to-CT™, Power SYBR™ Green |
| Type d’échantillon | Mammalian Cells |
| Méthode PCR | 1-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| À utiliser avec (application) | Gene Expression |
Invitrogen™ Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
| Format | Tube |
|---|---|
| Gamme de produits | Ambion |
| Marqueur ou colorant | Unconjugated |
| Contenu et stockage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
| Méthode de détection | SYBR |
|---|---|
| À utiliser avec (équipement) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | LD (Low DNA) |
| Type d’échantillon | Cells |
| Concentration | 20X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | RT-PCR |
| Polymérase | AmpliTaq Gold DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Gamme de produits | Cells-to-CT, SYBR |
| Transcriptase inverse | M-MLV |
| Temps de purification | 5 min. |
| Marqueur ou colorant | SYBR Green |
Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads
These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect, Platinum, SuperScript |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
|---|---|
| Format | Kit |
| Technologie d’isolement | ChargeSwitch™ Chemistry |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Type de produit | PCR Clean-Up Kit |
| Gamme de produits | ChargeSwitch |
| Type d’échantillon | PCR Products |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| Contenu et stockage | The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer. |
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Compatibilité à haut débit | Yes |
|---|---|
| Méthode de détection | TaqMan |
| Format | Kit |
| Technologie d’isolement | Direct cell lysis |
| Cible | cDNA |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | RNA |
| Méthode PCR | 2-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| Conditions d’expédition | Frozen, Dry Ice |
| Type de kit | Direct Cell Lysis RT-qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Direct PCR Master Mix |
| Type d’échantillon | Blood |
| En surplomb | Blunt |
| Méthode PCR | Direct PCR |
| Concentration | 2X |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Genotyping |
| Comprend l’étiquette ou le colorant | Yes |
| Polymérase | Phusion Blood II DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.
Invitrogen™ PureLink™ PCR Purification Binding Buffer B2
PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.
MilliporeSigma™ Calbiochem™ EMPIGEN™ BB Detergent, 30% Solution
Can be used as detergent for solubilization of membrane components