Direct PCR Reagents and Kits
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Résultats de la recherche filtrée
Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
| Volume d’élution max | 20 μL |
|---|---|
| Technologie d’isolement | Silica Spin Column |
| Type de produit | PCR Micro Kit |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Cloning,Standard PCR |
| Contenu et stockage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Volume de matériau de démarrage | 125 μL |
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
| Volume d’élution min | 5 μL |
| Durée de fonctionnement | 10 min. |
| Format | Spin Column |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Plage de séparation | 100 bp to 12 kb |
| Gamme de produits | PureLink |
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT |
| Matériau de départ | 10-100,000 Cells |
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
| Compatibilité à haut débit | High-throughput Compatible |
|---|---|
| Durée de fonctionnement | 5 min |
| Format | Kit |
| Technologie d’isolement | ChargeSwitch™ Chemistry |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Type de produit | PCR Clean-Up Kit |
| Gamme de produits | ChargeSwitch |
| Type d’échantillon | PCR Products |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| Contenu et stockage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Direct PCR Master Mix |
| Type d’échantillon | Blood |
| En surplomb | Blunt |
| Méthode PCR | Direct PCR |
| Concentration | 2X |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Genotyping |
| Comprend l’étiquette ou le colorant | Yes |
| Polymérase | Phusion Blood II DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
Applied Biosystems™ SequalPrep™ Normalization Plate Kit, 96-well
SequalPrep™ Normalization Plate Kit, 96-well
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
|---|---|
| Format | Kit |
| Technologie d’isolement | ChargeSwitch™ Chemistry |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Type de produit | PCR Clean-Up Kit |
| Gamme de produits | ChargeSwitch |
| Type d’échantillon | PCR Products |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
| Contenu et stockage | The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer. |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Compatibilité à haut débit | Yes |
|---|---|
| Méthode de détection | TaqMan |
| Format | Kit |
| Technologie d’isolement | Direct cell lysis |
| Cible | cDNA |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | RNA |
| Méthode PCR | 2-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| Conditions d’expédition | Frozen, Dry Ice |
| Type de kit | Direct Cell Lysis RT-qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
Invitrogen™ Cells-to-CT Bulk RT Reagents
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).
Applied Biosystems™ MicroSEQ™ 500 16S rDNA PCR Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The MicroSEQ™ 500 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. PCR-amplified products generated with this kit can be sequenced using the MicroSEQ™ 500 16S rDNA Sequencing Kit.