Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

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Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Prix et disponibilité
Spécifications

GC-Rich PCR Performance	Medium
Vitesse de réaction	Fast
Format de réaction	SuperMix or Master Mix
Type de produit	Direct PCR Master Mix
Type d’échantillon	Tissue
En surplomb	Blunt
Méthode PCR	Direct PCR
Concentration	2X
Conditions d’expédition	Wet or Dry Ice
À utiliser avec (application)	Genotyping,Hot-start PCR
Comprend l’étiquette ou le colorant	Yes
Polymérase	Phire Hot Start II DNA Polymerase
Hot Start	Built-In Hot Start
Matériau de départ	DNA

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Prix et disponibilité
Spécifications

GC-Rich PCR Performance	High
Concept écologique	Fewer resources used and less waste, Less hazardous
Méthode de détection	SYBR
Vitesse de réaction	7 min.
Type de produit	Fast Advanced Cells-to-Ct Kit
Type d’échantillon	Mammalian Cells
Cible de purification	Lysate
Méthode PCR	2-step RT-qPCR
Concentration	2X
Conditions d’expédition	Dry Ice
À utiliser avec (application)	Gene Expression
Compatibilité à haut débit	High-throughput Compatible
Type de réactif	Cell Lysis
Fidélité (par rapport à Taq)	2X
Format	Tube
Gamme de produits	Cells-to-CT™
Matériau de départ	10-100,000 Cells

Applied Biosystems™ SequalPrep™ Normalization Plate Kit, 96-well

SequalPrep™ Normalization Plate Kit, 96-well

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Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer

These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.

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Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents

This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.

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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Prix et disponibilité
Spécifications

Polymérase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Vitesse de réaction	Fast
Format de réaction	SuperMix or Master Mix
Type de produit	Direct PCR Master Mix
Hot Start	Built-In Hot Start
Type d’échantillon	Plant
En surplomb	Blunt
Méthode PCR	Direct PCR
Concentration	2X
Matériau de départ	DNA
Conditions d’expédition	Wet or Dry Ice
À utiliser avec (application)	Genotyping,Hot-start PCR

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Prix et disponibilité
Spécifications

Méthode de détection	Primer-probe
À utiliser avec (équipement)	7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Vitesse de réaction	Fast or Standard
Gamme de produits	CellsDirect™, Platinum™, SuperScript™
Type d’échantillon	Cells
Concentration	2X
À utiliser avec (application)	RT-PCR

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Prix et disponibilité
Spécifications

Méthode de détection	Primer-probe
À utiliser avec (équipement)	7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Vitesse de réaction	Fast or Standard
Gamme de produits	CellsDirect™, Platinum™, SuperScript™
Type d’échantillon	Cells
Concentration	2X
À utiliser avec (application)	RT-PCR

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

Prix et disponibilité
Spécifications

Méthode de détection	Primer-probe
À utiliser avec (équipement)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Type de produit	Multiplex Kit
Gamme de produits	SNaPshot™
Type d’échantillon	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension

MicroSEQ™ 500 16S rDNA PCR Kit

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Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

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Invitrogen™ SYBR™ Green Cells-to-C_T™ Control Kit

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-C_T™ Kits with sufficient reagents are provided for 100 reactions.

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Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

Prix et disponibilité

Invitrogen™ Cells-to-C_T™ Stop Solution

A component of the Cells-to-C_T™ Kits.

Prix et disponibilité

Direct PCR Reagents and Kits

Affiner les résultats

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Cells-to-CT™ Stop Solution Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More