Direct PCR Reagents and Kits
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Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, less hazardous |
| Méthode de détection | SYBR |
| Type de réactif | Cell Lysis |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | 7 min. |
| Format | Tube |
| Type de produit | 1-Step qPCR kit |
| Gamme de produits | Cells-to-CT™, Power SYBR™ Green |
| Type d’échantillon | Mammalian Cells |
| Méthode PCR | 1-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, Less hazardous |
| Méthode de détection | SYBR |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT™ |
| Matériau de départ | 10-100,000 Cells |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Applied Biosystems™ SequalPrep™ Normalization Plate Kit, 96-well
SequalPrep™ Normalization Plate Kit, 96-well
Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer
These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect™, Platinum™, SuperScript™ |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect™, Platinum™, SuperScript™ |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads
These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Concept écologique | REACH compliant, less waste |
|---|---|
| Méthode de détection | TaqMan |
| Technologie d’isolement | Direct cell lysis |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | RNA |
| Méthode PCR | 2-step RT-qPCR |
| Conditions d’expédition | Frozen, Dry Ice |
| Type de kit | Direct Cell Lysis RT-qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
| Compatibilité à haut débit | Yes |
| Format | Kit |
| Cible | cDNA |
| Matériau de départ | 10-100,000 Cells |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Invitrogen™ Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, Less hazardous |
| Méthode de détection | TaqMan |
| Type de réactif | Cell Lysis |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | 7 min. |
| Format | Tube |
| Type de produit | 1-Step qPCR kit |
| Gamme de produits | Cells-to-CT™, TaqMan™ |
| Type d’échantillon | Mammalian Cells |
| Méthode PCR | 1-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| À utiliser avec (application) | Gene Expression |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used, less waste, less hazardous |
| Méthode de détection | TaqMan |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT™ |
| Matériau de départ | 10-100,000 Cells |