Direct PCR Reagents and Kits
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Filtered Search Results
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.
| Shipping Condition | Frozen, Dry Ice |
|---|---|
| Target | cDNA |
| Content And Storage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
| Format | Kit |
| Sample Type | Mammalian Cells |
| Isolation Technology | Direct cell lysis |
| Kit Type | Direct Cell Lysis RT-qPCR |
| Purification Target | RNA |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | Yes |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
Thermo Scientific™ Template Generation System II Kit
Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.
Applied Biosystems™ Sequencing Chemistry Installation Kit for 3500xL Series Genetic Analyzer
The 3500 platform can run a wide variety of applications
Invitrogen™ Cells-to-CT Bulk RT Reagents
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).
Invitrogen™ PureLink™ PCR Purification Binding Buffer B2
PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.
Applied Biosystems™ CLA GlobalFiler™ PCR Amplification Kit
The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ Cells-to-CT™ Stop Solution
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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A component of the Cells-to-CT™ Kits.
Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
| Product Type | Multiplex Kit |
|---|---|
| For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
| Sample Type | DNA |
| Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
| Product Line | SNaPshot |
| Detection Method | Primer-probe |
Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.
Thermo Scientific™ Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Includes Label or Dye | Yes |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | LD (Low DNA) |
| Reverse Transcriptase | M-MLV |
| Purification Time | 5 min. |
| Concentration | 20X |
| For Use With (Application) | RT-PCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Cells |
| Reaction Speed | Standard |
| Label or Dye | SYBR Green |
| Hot Start | Built-In Hot Start |
| Product Line | Cells-to-CT, SYBR |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.