ELISA Substrates and Reagents
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Invitrogen™ eBioscience™ TMB Solution (1X)
Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.
Invitrogen™ ELISA Stop Solution
A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.
Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets
Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ Wash Buffer (25X)
Suitable for use with all ELISA assays
Invitrogen™ DynaLight™ Substrate with RapidGlow™ Enhancer
A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline
Invitrogen™ Luminescence Enhancer For Solution Assays, Sapphire-II™ Enhancer
Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.
Invitrogen™ ELISA-Light™ Immunoassay System with CSPD™ and Emerald-II™ Substrate/Enhancer Solution
Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.
Thermo Scientific™ PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
Invitrogen™ eBioscience™ ELISA/ELISPOT Diluent (5X)
Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative
Invitrogen™ ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution
Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.