ELISA Substrates and Reagents

ELISA Substrates and Reagents
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Thermo Scientificâ„¢ 1-Stepâ„¢ TMB ELISA Substrate Solutions
Thermo Scientificâ„¢ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Thermo Scientificâ„¢ QuantaBluâ„¢ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Thermo Scientificâ„¢ PNPP Substrates
Thermo Scientificâ„¢ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | Alkaline Phosphatase |
Detection Method | Colorimetric |
Thermo Scientificâ„¢ Pierceâ„¢ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientificâ„¢ 1-Stepâ„¢ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Invitrogenâ„¢ Super AquaBlue ELISA Substrate
Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.
Substrate Properties | Chemical Substrate |
---|---|
Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Invitrogenâ„¢ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Invitrogenâ„¢ DynaLightâ„¢ Substrate with RapidGlowâ„¢ Enhancer
A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline
Content And Storage | 1 amber bottle — Store at 2 to 8°C |
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Substrate Properties | Chemical Substrate |
Format | Bottle |
For Use With (Equipment) | Luminometer (Microplate) |
Form | Liquid |
Product Type | ELISA Substrate |
Sample Type | Cell Cultures, Whole Blood, Serum |
Substrate Type | Alkaline Phosphatase |
Assay Sensitivity | 1 pg/mL |
High-throughput Compatibility | High-throughput Compatible |
Test Time | 2 min. |
Product Line | DynaLightâ„¢, NovaBrightâ„¢, RapidGlowâ„¢ |
Detection Method | Chemiluminescence |
Invitrogenâ„¢ ELISA/ELISPOT Coating Buffer Powder
Designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.
Invitrogenâ„¢ eBioscienceâ„¢ ELISA Wash Buffer, 1L packets
Suitable for washing ELISA plate microwells. eBioscienceâ„¢ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
Invitrogenâ„¢ eBioscienceâ„¢ ELISA/ELISPOT Diluent (5X)
Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative
Invitrogenâ„¢ eBioscienceâ„¢ TMB Solution (1X)
Recommended for ELISA Ready-SET-Go!â„¢ kits. eBioscienceâ„¢ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.
Thermo Scientificâ„¢ SuperSignalâ„¢ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Content And Storage | Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature. |
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Substrate Properties | Chemical Substrate |
Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Product Line | SuperSignalâ„¢ |
Detection Method | Chemiluminescence |
Thermo Scientificâ„¢ ELISA Wash Buffer (30X)
Concentrated wash buffer for efficient use of storage in the lab