ELISA Substrates and Reagents
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Filtered Search Results
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ 1-Step™ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Detection Method | Colorimetric |
Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets
Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Thermo Scientific™ ELISA Wash Buffer (30X)
Concentrated wash buffer for efficient use of storage in the lab
Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)
The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
| Content And Storage | Store the product at 4°C. |
|---|---|
| Format | Bottle |
| Form | Liquid |
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Product Line | CDP-Star, NovaBright |
Invitrogen™ ELISA-LIGHT W/CDP-STAR/SII EAC
Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.
Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
| Shipping Condition | Wet Ice |
|---|
Invitrogen™ DynaLight™ Substrate with RapidGlow™ Enhancer
A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline
| Content And Storage | 1 amber bottle — Store at 2 to 8°C |
|---|---|
| Substrate Properties | Chemical Substrate |
| Format | Bottle |
| For Use With (Equipment) | Luminometer (Microplate) |
| Form | Liquid |
| Product Type | ELISA Substrate |
| Sample Type | Cell Cultures, Whole Blood, Serum |
| Substrate Type | Alkaline Phosphatase |
| Assay Sensitivity | 1 pg/mL |
| High-throughput Compatibility | High-throughput Compatible |
| Test Time | 2 min. |
| Product Line | DynaLight, NovaBright, RapidGlow |
| Detection Method | Chemiluminescence |
Invitrogen™ Luminescence Enhancer For Solution Assays, Sapphire-II™ Enhancer
Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.
Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.