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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 65 results
1

Invitrogen™ Wash Buffer (25X)  DFS Item

Suitable for use with all ELISA assays

Shipping Condition Wet Ice
2

Invitrogen™ DynaLight™ Substrate with RapidGlow™ Enhancer  DFS Item

A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline

Content And Storage 1 amber bottle — Store at 2 to 8°C
Substrate Properties Chemical Substrate
Format Bottle
For Use With (Equipment) Luminometer (Microplate)
Form Liquid
Product Type ELISA Substrate
Sample Type Cell Cultures, Whole Blood, Serum
Substrate Type Alkaline Phosphatase
Assay Sensitivity 1 pg/mL
High-throughput Compatibility High-throughput Compatible
Test Time 2 min.
Product Line DynaLight, NovaBright, RapidGlow
Detection Method Chemiluminescence
3

Invitrogen™ Luminescence Enhancer For Solution Assays, Sapphire-II™ Enhancer  DFS Item

Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.

4

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

5

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

6

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
7

Invitrogen™ Assay Buffer (10X)  DFS Item

Used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates.

8

Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate with Enhancer
Substrate Type Alkaline Phosphatase
Quantity 100 mL
Product Line CDP-Star, NovaBright
Detection Method Chemiluminescence
9

Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)  DFS Item

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Chemiluminescence
10

Invitrogen™ ELISA Stop Solution  DFS Item

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Shipping Condition Ambient
Content And Storage Room Temperature
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
11

Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

13

Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer  DFS Item

The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.

14

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

15

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence