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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 49 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate with Enhancer
Substrate Type Alkaline Phosphatase
Quantity 100 mL
Product Line CDP-Star, NovaBright
Detection Method Chemiluminescence
4

Thermo Scientific™ ELISA Wash Buffer (30X)

Concentrated wash buffer for efficient use of storage in the lab

5

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

6

Invitrogen™ eBioscience™ TMB Solution (1X)

Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.

7

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

8

Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

9

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

10

Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

11

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

12

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

13

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

14

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
15

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence